Russian veterinary journal Russian veterinary journal Российский ветеринарный журнал 2500-4379 67180 ДЕРМАТОЛОГИЯ DERMATOLOGY ДЕРМАТОЛОГИЯ Evaluation of clonality from multiple anatomic sites in canine epitheliotropic T cell lymphoma Оценка клональности эпителиотропной Т-клеточной лимфомы собак разной анатомической локализации Китинг М. Келли Keating M. Kelly Розенкранц Вейн С. Rosenkrantz Wayne S. Келлер Стефан М. Keller Stefan M. Моор Питер Ф. Moore Peter F. Ветеринарная клиника Тастин США Veterinary clinic Tustin United States of America Ветеринарная клиника Тастин США Veterinary clinic Tustin United States of America Университет Калифорнии-Дэвис Дэвис США University of California-Davis Davis United States of America Университет Калифорнии-Дэвис Дэвис США University of California-Davis Davis United States of America 07 07 2023 07 07 2023 2023 2 32 41 05 07 2023 https://logospress.editorum.ru/en/nauka/article/67180/view

Предпосылки. Полагают, что эпителиотропная кожная Т-клеточная лимфома собак (эКТКЛ) представляет собой заболевание, гомологичное фунгоидной гранулеме (ФГ) у человека. При ФГ у людей опухолевые клетки фенотипически соответствуют резидентным эффекторным Т-клеткам памяти, популяции, сохраняющейся в тканях длительное время без циркуляции. У собак с эКТКЛ часто появляются очаги в разных местах, что вызывает вопрос, происходит ли новообразование из одной субпопуляции Т-клеток или нет. Цели. Описать перестройки гена антигенного рецептора лимфоцитов из кожи и крови собак с эКТКЛ, чтобы установить, идентичны ли клоны опухоли. Животные. 14 собак с эКТКЛ. Материалы и методы. Гистологическое и иммуногистохимическое исследование, ПЦР для обнаружения перестройки антигенного рецептора (PARR) Т-клеточного рецептора гамма (TRG) различных биоптатов кожи и крови. Результаты. Все биоптаты кожи содержали неопластические лимфоциты, содержащие кластер дифференциации (CD)3. У отдельных собак все биоптаты кожи показали идентичные профили клональности TRG, что дает основания предполагать присутствие одного опухолевого клона во всех очагах. В крови соответствующий клон обнаружен у 6 из 14 собак, уникальный клон выявлен у 9 из 14 собак, и у 2 из 14 не было обнаружено клона. Выводы. Эти данные показывают, что очаги эКТКЛ собак в нескольких локализациях содержат один клон опухоли, неопластические лимфоциты не остаются неподвижно в коже, а могут циркулировать в крови, в коже и крови можно обнаружить различающиеся клоны, и циркулирующие неопластические клетки не обязательно сопровождаются лимфоцитозом.

Background. Canine epitheliotropic cutaneous T-cell lymphoma (eCTCL) is thought to represent a disease homologue to human mycosis fungoides (MF). In human MF, neoplastic cells are phenotypically consistent with resident effector memory T cells, a population that remains for an extended period within tissue without circulating. Dogs with eCTCL often present with lesions in multiple locations, raising the question of whether the neoplasm is of the same T-cell subpopulation or not. Objectives: To characterize the antigen receptor gene rearrangements of lymphocytes from skin and blood of dogs with eCTCL to determine if neoplastic clones are identical. Animals. Fourteen dogs with eCTCL. Materials and methods. Histological and immunohistochemical examination, and PCR for antigen receptor rearrangement (PARR) for T-cell receptor gamma (TRG) performed on multiple cutaneous biopsy samples and blood. Results. All skin biopsies contained cluster of differentiation (CD)3-positive neoplastic lymphocytes. Within individual dogs, all skin biopsies revealed identical TRG clonality profiles, suggesting that the same neoplastic clone was present in all sites. In the blood, a matching clone was found in six of 14 dogs, a unique clone was observed in nine of 14 dogs, and no clone was detected in two of 14 dogs. Conclusions. These findings show that canine eCTCL lesions in multiple locations harbour the same neoplastic clone, neoplastic lymphocytes do not remain fixed to the skin and instead can circulate via blood, differing clones can be identified in skin versus blood, and circulating neoplastic cells can be detected without lymphocytosis.

 эпителиотропная кожная Т-клеточная лимфома собаки клональность опухоли перестройка гена антигенного рецептора лимфоцитов epitheliotropic cutaneous T-cell lymphoma dogs tumor clonality antigen receptor gene rearrangements of lymphocytes

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