employee
Moskva, Moscow, Russian Federation
employee
Moskva, Moscow, Russian Federation
employee
Moscow, Russian Federation
employee
Russian Federation
employee
Moscow, Russian Federation
employee
Russian Federation
employee
Moskva, Moscow, Russian Federation
employee
Russian Federation
employee
Russian Federation
employee
Russian Federation
employee
Moskva, Moscow, Russian Federation
GRNTI 68.41 Ветеринария
A pharmacokinetic study of the absorption into the bloodstream, bioavailability and excretion of Gamavit from the body after intramuscular administration to laboratory mini-pigs was conducted. Quantitative determination was carried out by HPLC using a fluorimetric detector, for which Gamavit was labeled with Cy5 dye, which was then used for mini-pigs inoculation. The developed methods for determining Gamavit in the blood and feces were validated according to the following validation parameters: selectivity, calibration curve, accuracy, precision, limit of quantitative determination, sample transfer, and sample stability. The confirmed analytical range of the method for Gamavit detection in blood plasma and feces was 1.00…50.0 mcg/ml. Maximum concentration of Gamavit in the blood of mini-pigs after a single intramuscular injection was 30.97 mcg/ml and was reached on average 15 minutes after administration. 24 hours following administration, Gamavit was still detected in the blood in insignificant amounts. The average half-life of Gamavit in the blood is 8.64±3.50 hours. After administration at a dose of 0.1 ml/kg, the clearance of the drug is 1.27 l/kg * h, the excretion rate at an effective concentration of 30 mg/l is 38 mg/kg*h, and the maintenance dose when using the drug 1 time a day is 0.9…1.0 ml. The detection of the label in the feces of the studied animals indicates that one of the ways Gamavit removal is excretion with the help of bile acids, as well as partial excretion with feces.
Gamavit, absorption into the bloodstream, mini-pigs, blood plasma, feces, veterinary drug
1. Annikov, V.V. Effektivnost' Gamavita pri provedenii degel'mintizacii kotyat i schenkov / V.V. Annikov, A.V. Krasnikov, A.V. Sanin // Veterinarnaya klinika. - 2018. - № 5. - S. 24-25.
2. Annikov, V.V. Effektivnost' gamavita pri terapii borrelioza u sobak / V.V. Annikov, L.V. Annikova, A.V. Sanin, A.N. Narovlyanskiy, A.V. Pronin, V.Yu. Sanina, T.N. Kozhevnikova // Veterinariya Kubani. - 2018. - № 4 - S. 15-18.
3. Vishnevskaya, T.Ya. Sravnitel'nyy analiz gematologicheskih pokazateley krovi pri stresse i ego immunokorrekciya preparatom gamavit / T.Ya. Vishnevskaya, L.L. Abramova // V sb.: «Aktual'nye voprosy veterinarnoy mediciny», Materialy odinnadcatoy Sibirskoy veterinarnoy konferencii, 2012 - S. 59-60.
4. Glagoleva, T.I. Ferroglyukin i gamavit v korrekcii antiagregacionnyh svoystv sosudov u novorozhdennyh telyat s deficitom zheleza / T.I. Glagoleva, S.Yu. Zavalishina, I.N. Medvedev // Uspehi sovremennogo estestvoznaniya. - 2013. - № 5. - S. 17.
5. Grigor'eva, E.A. Vozdeystvie preparata Gamavit na aktivnost' estestvennyh killernyh kletok / E.A. Grigor'eva, A.V. Pronin, A.V. Sanin, A.N. Narovlyanskiy, T.Yu. Timofeeva, V.Yu. Sanina, T.N. Stepanova, E.V. Gerasimova, A.M. Ivanova, T.N. Kozhevnikova // Veterinariya Kubani. - 2016. - №4. - S. 27-28.
6. Grishina, E.A. Biohimicheskoe obosnovanie primeneniya kompleksnoy terapii v ostroy faze eksperimental'nyh gel'mintozov zhivotnyh / E.A. Grishina, A.A. Erovichenkov // Zhurnal infektologii. - 2017. - T.9. - №3. - S. 32-39.
7. Zaripova, T.E. Ostroe otravlenie u sobaki: klinicheskiy sluchay / T.E. Zaripova, E.A. Bakulina, S.E. Zhavnis // Veterinarnaya klinika. - 2017. - № 8. - C. 10-12.
8. Leonard, R.A. Vliyanie gamavita, fosprenila i maksidina na ryad biohimicheskih pokazateley krovi sobak, bol'nyh piroplazmozom / R.A. Leonard // Veterinarnaya klinika. - 2006. - № 3. C. 2-5.
9. Leonard, R.A. Ocenka effektivnosti preparata Gamavit pri korrekcii renal'noy anemii u koshek s HBP: kontroliruemoe randomizirovannoe issledovanie / R.A. Leonard, T.E. Zaripova, A.N. Balahonov, N.Yu. Vdovina, A.N. Narovlyanskiy, A.V. Pronin, A.V. Sanin // Rossiyskiy veterinarnyy zhurnal. - 2020. - № 5. - S. 8-20. DOI:https://doi.org/10.32416/2500-4379-2020-5-8-20
10. Liberman, E.L. Opyt primeneniya gamavita pri lechenii kroveparazitarnyh bolezney severnyh oleney / E.L. Liberman, H. Georgiu, V.V. Belimenko // Rossiyskiy veterinarnyy zhurnal. SHZh. - 2014. - № 4. - S. 31-33.
11. Obryvin, V.N. Vliyanie preparatov gamavit i gala-vet na toksicheskiy immunodeficit u belyh krys / V.N. Obryvin, G.A. Zhorov, P.N. Rubchenkov // Veterinarnaya patologiya. - 2008. - № 3. - S. 119-125.
12. Pereslegina, I.O. Effektivnost' primeneniya gamavita pri toksokaroze u koshek / I.O. Pereslegina, T.N. Kozhevnikova // Izvestiya Orenburgskogo agrarnogo universiteta. - 2018. - № 6(74). - S. 162-163.
13. Pereslegina, I.O. Klinicheskie sluchai hronicheskogo otravleniya koshek: opyt primeneniya preparata Gamavit / I.O. Pereslegina, T.S. Dubrovina, T.Yu. Klincova, S.N. Zotova, T.N. Kozhevnikova, A.V. Sanin // Veterinariya Kubani. - 2017. - № 5. - S. 23-26.
14. Pereslegina, I.O. Effektivnost' gamavita pri otravlenii koshki ivermektinom / I.O. Pereslegina // Shkola nauki. - 2018. - № 6. - S.14-15.
15. Rybakova, A.V. Karlikovye svin'i kak ob'ekt doklinicheskih issledovaniy / A.V. Rybakova, M.A. Kovaleva, A.V. Kalatanova, G.V. Vanatiev, M.N. Makarova // Mezhdunarodnyy vestnik veterinarii. - SPb. - 2016. - № 3. - S. 168-176.
16. Samoylova, E.S. Iz opyta primeneniya antioksidantov v kompleksnoy sheme lecheniya babezioza sobak. / E.S. Samoylova, M.A. Derho // Veterinarnyy vrach. - 2009. - № 1. - S. 13-16.
17. Sanin, A.V. Gamavit - effektivnoe sredstvo pri ekstrakorpuskulyarnyh anemiyah / A.V. Sanin // Veterinarnaya klinika. - 2009. - № 4. - S. 16-19.
18. Sanin, A.V. Osobennosti primeneniya immunomodulyatorov pri parazitarnyh invaziyah / A.V. Sanin, O.Yu. Sosnovskaya, V.Yu. Sanina, T.N. Kozhevnikova, I.K. Vasil'ev, A.N. Narovlyanskiy, A.V. Pronin // Veterinariya Kubani. - 2010. - № 2. - S.15-18.
19. Sanin, A.V. Protektivnyy effekt Gamavita pri ostroy intoksikacii, vyzvannoy chetyrehhloristym uglerodom v eksperimente / A.V. Sanin, S.V. Ozherelkov, O.Yu. Sosnovskaya, A.N. Narovlyanskiy, A.V. Pronin // Rossiyskiy veterinarnyy zhurnal. SHZh. - 2015. - № 2. - S. 40-42.
20. Sanin, A.V. Gamavit povyshaet effektivnost' terapii gemobartonelleza (gemoplazmoza) u koshek: kontroliruemoe issledovanie / A.V. Sanin, A.N. Narovlyanskiy, A.V. Pronin, T.N. Kozhevnikova, A.D. Agafonova, L.V. Annikova, V.V. Annikov // Rossiyskiy Veterinarnyy Zhurnal. - 2017. - № 3. - S. 28-32.
21. Sanin, A.V. Gamavit dlya korrekcii toksicheskoy gemoliticheskoy anemii i stimulyacii eritropoeza / A.V. Sanin, A.N. Narovlyanskiy, A.V. Pronin, T.N., Kozhevnikova O.Yu. Sosnovskaya, S.E. Zhavnis, S.V. Ozherelkov, D.A. Klimova // Veterinariya. - 2018. - № 10. - S. 54-59.
22. Klöcker, N. Covalent labeling of nucleic acids DOI:https://doi.org/10.1039/D0CS00600A (Review Article) / N. Klöcker, F.P. Weissenboeck, A. Rentmeister // Chem. Soc. Rev. - 2020. - No. 49. - pp. 8749-8773.
23. Nikić, I. Labeling proteins on live mammalian cells using click chemistry / I Nikić., J. Kang, G. Girona, Aramburu I.V., Lemke E.A. // Nat Protoc. - 2015. - No. 10. - pp. 780-791. https://doi.org/10.1038/nprot.2015.045
