Peoples’ Friendship University of Russia (Department of Veterinary Medicine, Professor)
Russian Federation
Russian Federation
GRNTI 68.41 Ветеринария
Cryptosporidiosis is a widespread parasitic (protozoal) disease. to which more than 170 species of animals are susceptible. Purpose of the study. To study the spread of cryptosporidiosis of calves in the conditions of private farmsteads in some areas on the territory of the Karachay-Cherkess Republic. Materials and methods. Fecal samples from 55 calves (clinically healthy and sick, with signs of diarrhea) aged 3 to 50 days were examined for the presence of the causative agent of cryptosporidiosis using the native smear method. The finished smears were fixed according to the Nikiforov method, stained with carbolic fuchsin according to Ziehl–Neelsen and examined under a binocular light microscope «Micromed-3» with an immersion lens. Cryptosporidium oocysts acquire a rich pink-red color when stained by Ziehl–Neelsen. The final diagnosis was established based on the detection of oocysts characteristic of this invasion. Cryptosporidium was identified using the «Determinant of parasitic protozoa» of Krylov M.V. and the atlas of Vershinin I.I. Results of the study. Microscopy of smears of faecal samples from 55 calves revealed cryptosporidium oocysts in 29 smears, thus, the extent of invasion (EI) was 52.7 %.
cryptosporidiosis, calves, diagnostics, native smear method, Zil-Nielsen staining, oocysts, Cryptosporidium parvum
1. Akbaev, M.Sh. K metodike laboratornoy diagnostiki kriptosporidoza telyat / M.Sh. Akbaev, E.V. Petrovich, R.M. Akbaev // Sbornik statey Mezhdunarodnoy nauchno-prakticheskoy konferencii, posvyaschennoy 100-letiyu so dnya rozhdeniya zasluzhennogo deyatelya nauki RSFSR, doktora veterinarnyh nauk, professora Abuladze Konstantina Ivanovicha i doktora veterinarnyh nauk professora Kolobolotskogo Georgiya Vasil'evicha, Moskva, 15-16 maya 2012 goda. - Moskva: Vserossiyskiy nauchno-issledovatel'skiy institut fundamental'noy i prikladnoy parazitologii zhivotnyh i rasteniy im. K.I. Skryabina, 2012. - S. 128-132. - EDN VEDSSZ.
2. Akbaev, R.M. K voprosu o korrektnom upotreblenii latinizirovannyh terminov v parazitologii / R. M. Akbaev, N. V. Babichev // Rossiyskiy veterinarnyy zhurnal. - 2021. - № 2. - S. 5-12. - DOIhttps://doi.org/10.32416/2500-4379-2021-2-5-12. - EDN SQQAUM.
3. Akbaev, R.M. Ispol'zovanie terminov v veterinarnoy parazitologii : Uchebno-metodicheskoe posobie po chteniyu i upotrebleniyu parazitologicheskih terminov dlya obuchayuschihsya v veterinarnyh i biologicheskih VUZah, po napravleniyu 36.05.01. «Veterinariya»; 36.03.01. «Veterinarno-sanitarnaya ekspertiza» i 06.03.01. «Biologiya» / R. M. Akbaev, N. V. Babichev. - M.: Moskovskaya gosudarstvennaya akademiya veterinarnoy mediciny i biotehnologii - MVA im. K.I. Skryabina, 2021. - 36 s. - ISBN 978-5-6047469-0-5. - EDN PQRGUU.
4. Akbaev, R.M. Bovikolez krupnogo rogatogo skota v zhivotnovodcheskih hozyaystvah Moskovskoy oblasti / R.M. Akbaev, N.V. Pugovkina // Rossiyskiy veterinarnyy zhurnal. - 2017. - № 1. - S. 10-13. - EDN XXYQUN.
5. Bayramgulova, G.R. Osobennosti rasprostraneniya kriptosporidioza telyat v Bashkirskom Zaural'e / G.R. Bayramgulova, Z.I. Syrlybaeva, R.N. Samigullin //Agrarnyy vestnik Urala. - 2008. - №. 7.S. 56-57.
6. Beyer, T.V. Kishechnyy kriptosporidioz v rannem vozraste i ego posledstviya / T.V. Beyer, N.V. Sidorenko, O.V. Anackaya // Medicinskaya parazitologiya i parazitarnye bolezni. - 2006. - № 1. - S. 3-7.
7. Bochkarev, I.I. Kriptosporidioz telyat / I.I. Bochkarev // Profilaktika virusnyh i protozoynyh bolezney molodnyaka KRS: Metodicheskaya rekomendaciya. - Novosibirsk, 1991. - 23s.
8. Vershinin I.I. Kokcidiozy zhivotnyh i ih differencial'naya diagnostika. - Ekaterinburg. 1996. 264s.
9. Goncharov, D.B. Kriptosporidioz: sovremennye predstavleniya o patogeneze i rezistentnosti / D.B. Goncharov, N.V. Kobec, E.V. Nagurskaya // Medicinskaya parazitologiya i parazitarnye bolezni. - 2007. - №1. - S. 56-60.
10. Gurbanova, T.F. Sravnitel'nyy analiz zarazhennosti kriptosporidiyami (Coccidia, Eimeriida, Cryptosporidium) rogatogo skota i gryzunov v raznyh ekosistemah Azerbaydzhana / T.F. Gurbanova // Vestnik Moskovskogo gosudarstvennogo oblastnogo universiteta. Seriya: Estestvennye nauki. - 2015. - №. 4. - S. 22-28.
11. Kirillov, E.G. Epizootologiya kriptosporidioza v respublike Tatarstan / E.G. Kirillov, D.G. Latypov // Uchenye zapiski Kazanskoy gosudarstvennoy akademii veterinarnoy mediciny im. NE Baumana. - 2015. - №. 223. - S. 93-95.
12. Krylov, M.V. Opredelitel' paraziticheskih prosteyshih: cheloveka, domashnih zhivotnyh i sel'skohozyaystvennyh rasteniy / M.V. Krylov. - M.: Zoologicheskiy in-t RAN, 1996. - 603 s.
13. Metody bor'by s gnusom i iksodovymi kleschami v hozyaystvah Ryazanskoy oblasti / M.Sh. Akbaev, F.I. Vasilevich, R.M. Akbaev [i dr.] // Veterinariya. - 2004. - № 10. - S. 29-31. - EDN ODEJOF.
14. Musaeva, M.N. Kriptosporidioz pri immunodeficite u novorozhdennyh telyat / M.N. Musaeva, N.R. Budulov, S.Sh. Abdulmagomedov, Z.G. Musaev //Rossiyskiy parazitologicheskiy zhurnal. - 2013. - №. 3. - S. 64-66.
15. Parazitarnye zoonozy / M.V. Yakubovskiy [i dr.]; Pod. red. M.V. Yakubovskogo. - Minsk: Nasha Ideya, 2012. - 384 s.
16. Pepelyaeva, O.P. Kriptosporidioz (obzor) / O.P. Pepelyaeva, M.V. Yakubovskiy // Ekologiya i zhivotnyy mir. - 2013. - №. 1. - S. 41-46.
17. Petrovich, E.V. Izuchenie epizootologicheskih aspektov kriptosporidioza telyat v Moskovskoy oblasti / E.V. Petrovich, M.Sh. Akbaev //Rossiyskiy parazitologicheskiy zhurnal. - 2011. - №. 2. - S. 68-71.
18. Suhomlinov, V.N. Epizooticheskaya situaciya po kriptosporidiozu krupnogo rogatogo skota v skotovodcheskih hozyaystvah Belgorodskoy oblasti / V.N. Suhomlinov, O.A. Manzhurina, B.V. Romashov, A.M. Skogoreva // Teoriya i praktika parazitarnyh bolezney zhivotnyh. - 2014. - №. 15. - S. 87-90.
19. Uspenskiy, A.V. Vypolnenie koordinacionnyh planov nauchnyh issledovaniy v oblasti veterinarnoy parazitologii / A.V. Uspenskiy, E.I. Malahova, T.A. Ershova // Rossiyskiy parazitologicheskiy zhurnal. - 2013. - №. 2. - S. 48-53.
20. Chistenko, G.N. Kriptosporidioz / G.N. Chistenko // Veterinarnaya medicina. - 2001. - № 2. - S. 131-134.
21. Dolbin, D.A. Resistance of helminth eggs to unfavorable physical, chemical and biological factors of the environment (Literature review) / D.A. Dolbin, R.Z. Khayrullin // Russian Journal of Parasitology. - 2017. - Vol. 39. - No. 1. - pp. 14-19.
22. Holzhausen, I. Distribution of Cryptosporidium parvum gp 60 subtypes in calf herds of Saxony, Germany / I. Holzhausen, M. Lendner, F. Göhring, I. Steinhöfel, A. Daugschies // Parasitology research. - 2019. - Vol. 118. - No. 5. - pp. 1549-1558.
23. Hoque, S. High occurrence of zoonotic subtypes of Cryptosporidium parvum in Cypriot dairy farms / S. Hoque, D.E. Mavrides, P. Pinto, S. Costas, N. Begum, C. Azevedo-Ribeiro, A.D. Tsaousis // Microorganisms. - 2022. - Vol. 10. - No. 3. - pp. 531.
24. Kaupke, A. Population genetics of Cryptosporidium parvum subtypes in cattle in Poland: the geographical change of strain prevalence and circulation over time / A. Kaupke, A. Rzeżutka // BMC Veterinary Research. - 2022. - Vol. 18. - No. 1. - pp. 1-12.
25. Uga, S. Prevalence of Cryptosporidium parvum infection and pattern of oocyst shedding in calves in Japan / S. Uga, J. Matsuo, E. Kono, K. Kimura, M. Inoue, S.K. Rai, K. Ono // Veterinary parasitology. - 2000. - Vol. 94. - No. 1-2. - pp. 27-32.
26. Rieux, A. Molecular characterization of Cryptosporidium isolates from high-excreting young dairy calves in dairy cattle herds in Western France / A. Rieux, C. Chartier, I. Pors, A. Delafosse, C. Paraud // Parasitology research. - 2013. - Vol. 112. - No. 10. - pp. 3423-3431.
27. Soba, B. Genetic classification of Cryptosporidium isolates from humans and calves in Slovenia / B. Soba, J. Logar // Parasitology. - 2008. - Vol. 135. - No. 11. - pp. 1263-1270.
